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DNA Electrophoresis

Posted on Feb 08 2013 by Dale Hancock

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Experiment Overview

DNA electrophoresis is a fundamental technique used in Molecular Biology to separate DNA fragments by size. In this experiment first year students digest lambda DNA with an unknown restriction enzyme (one of a possible 4) and separate the products by agarose gel electrophoresis. Based on the number and size of the fragments obtained they can identify their unknown enzyme. The sites on lambda for all 4 possible unknown restriction enzymes are provided so the expected fragment sizes for each possible digest can be predicted.

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